4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid HEPES
Alias: N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
CAS NO.: 7365-45-9
Molecular formula: C8H18N2O4S
Sepcification Standard
Appearance White Crystalline Powder
Assay % ≥99.5
Solubility(1m Aq) Clear,colorless solution
PH(1% aq) 5.0-6.5
Heavy Metals (PPM) ≤10
Loss On Drying % ≤1.0
UV 260nm(1m aq) ≤0.1
UV 280nm(1m aq) ≤0.07
Fe Iron ppm ≤5
Sulfate Ion % ≤0.05
Purpose: 4- hydroxyethyl piperazine ethyl sulfonic acid is a biological buffer; The reaction of the separation and analysis of RNA component buffer, buffer, hybridization buffer in advance; Used for RNA and T4RNA ligase 3'- terminal marking; Used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kits. To uninfected cells function. It is a kind of hydrogen ion buffer, can control the scope of the pH value of the constant for a long time. Use the final concentration of 10-50 tendency for L, general culture medium containing 20 tendency LHEPES buffering capacity could be achieved.